Part:BBa_K5232012

Usage and Biology

It has a sequence complementary to hsa-miR-375-5p on the 5' end side and a sequence complementary to template0 on the 3' end side.

Figure 1.

Figure 2.

The larger this ratio, the more clearly the target concentration can be distinguished. For Biomarker 2, when the condition was 10-5, that is, when the hybridize length between template and target was 10 bp, and the hybridize length between template and helper was 5 bp, the ratio was the largest.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Qing, Z., Feng, C.,Feng, X., Yongxi, Z., & Chunhai, F. (2014). Target-Triggered Three-Way Junction Structure and Polymerase/Nicking Enzyme Synergetic Isothermal Quadratic DNA Machine for Highly Specific, One-Step, and Rapid MicroRNA Detection at Attomolar Level. Anal. Chem. 2014, 86, 16, 8098-8105. https://doi-org.utokyo.idm.oclc.org/10.1021/ac501038r